Intermediate
Part:BBa_K3589108:Design
Designed by: Yannik Schermer Group: iGEM20_TU_Kaiserslautern (2020-10-23)
Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419
Illegal PstI site found at 1496 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419
Illegal PstI site found at 1496 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1055
Illegal BglII site found at 2014
Illegal BamHI site found at 1394 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419
Illegal PstI site found at 1496 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419
Illegal PstI site found at 1496 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
he natural secretion signal of Botrytis aclada was removed. This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Chlamydomonas reinhardtii.